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Research Spending & Results

Award Detail

Doing Business As Name:Murray State University
  • Alexey Arkov
  • (270) 809-6053
Award Date:07/16/2021
Estimated Total Award Amount: $ 822,418
Funds Obligated to Date: $ 333,089
  • FY 2021=$333,089
Start Date:08/01/2021
End Date:07/31/2024
Transaction Type:Grant
Awarding Agency Code:4900
Funding Agency Code:4900
CFDA Number:47.074
Primary Program Source:040100 NSF RESEARCH & RELATED ACTIVIT
Award Title or Description:RUI: Biochemical and genetic analysis of conserved molecular scaffold Tudor complex required for germ cell specification in Drosophila
Federal Award ID Number:2130162
DUNS ID:041980632
Parent DUNS ID:041980632
Program:Cellular Dynamics and Function
Program Officer:
  • Charles Cunningham
  • (703) 292-2283

Awardee Location

Street:328 Wells Hall
Awardee Cong. District:01

Primary Place of Performance

Organization Name:Murray State University
Street:328 Wells Hall
Cong. District:01

Abstract at Time of Award

Cellular organization is critically dependent on the self-assembly of various membraneless organelles inside the cell. However, the principles and molecular mechanisms that drive and regulate the assembly of these organelles and define their functions are poorly understood. This project integrates cutting-edge genetics and molecular quantitative approaches to provide major insights into the mechanisms responsible for the assembly and function of evolutionarily conserved membraneless organelles assembled in germ cells of model organism Drosophila (germ granules). These cells give rise to egg and sperm cells, and therefore, are responsible for continuity of life. This research will be integrated into several cell and genetics undergraduate courses and will directly engage undergraduate and graduate students in quantitative cell and molecular biology training. Germ granules show the properties of soft condensed matter and they often change their morphology during developmental transitions. Recent research identified an evolutionarily conserved germ granule multisubunit complex assembled on the large scaffold Tudor protein. Tudor scaffold has 11 protein-protein interaction modules (Tudor domains) and Tudor complex components are required for germ cell specification. The goal of this project is to provide molecular understanding of how this multisubunit Tudor scaffold complex is assembled using purified components and quantitative biochemical approaches. In addition, using super-resolution microscopy, it will be determined how different components of the complex are assembled into large germ granules in vivo. Furthermore, to understand the function of Tudor complex, enzymatic activities of its components upon the complex assembly will be characterized and the significance of the complex for germ granule formation, morphology and germ cell specification will be determined by mutational approaches. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

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