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Minimize RSR Award Detail

Research Spending & Results

Award Detail

Awardee:TRINITY COLLEGE
Doing Business As Name:Trinity College
PD/PI:
  • Michelle L Kovarik
  • (860) 297-5275
  • Michelle.kovarik@trincoll.edu
Award Date:07/29/2021
Estimated Total Award Amount: $ 293,514
Funds Obligated to Date: $ 293,514
  • FY 2021=$293,514
Start Date:09/01/2021
End Date:08/31/2024
Transaction Type:Grant
Agency:NSF
Awarding Agency Code:4900
Funding Agency Code:4900
CFDA Number:47.074
Primary Program Source:040100 NSF RESEARCH & RELATED ACTIVIT
Award Title or Description:RUI: Microelectrophoretic and Statistical Tools for Studies of Kinase- and ROS-Based Signaling in Dictyostelium discoideum
Federal Award ID Number:2126177
DUNS ID:069259950
Parent DUNS ID:069259950
Program:Cellular Dynamics and Function
Program Officer:
  • Steve Clouse
  • (703) 292-8440
  • sclouse@nsf.gov

Awardee Location

Street:300 Summit Street
City:Hartford
State:CT
ZIP:06106-3100
County:Hartford
Country:US
Awardee Cong. District:01

Primary Place of Performance

Organization Name:Trinity College
Street:300 Summit Street
City:Hartford
State:CT
ZIP:06106-3100
County:Hartford
Country:US
Cong. District:01

Abstract at Time of Award

Living organisms use a diversity of molecules to communicate information within and between cells, and scientists are still investigating the interactions between these molecules. This project will produce simultaneous measurements of both large proteins and small reactive, oxygen-containing molecules in groups of cells and in individual cells to better understand the interactions between these molecular signals. The project will also develop mathematical tools to analyze the data collected from these experiments and make these tools available to researchers doing similar work. Finally, this project will engage undergraduates and high school students in interdisciplinary research involving cell signaling, separations chemistry, microfluidics, and mathematics. The amoeba Dictyostelium discoideum undergoes a social life cycle, in which individual cells aggregate and differentiate to form a superorganism. Cell signaling during this process involves both protein kinases and reactive oxygen species, but the relationship between these interacting signaling nodes remains unclear. Through microelectrophoretic separations of peptide substrates and fluorogenic indicators, this project will simultaneously measure protein kinase B activity and superoxide concentrations in cells. In addition to characterizing these molecules in cell lysates, this project will also produce single-cell measurements using custom-designed microfluidic devices and novel mathematical strategies for data analysis. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

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