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Award Detail

Doing Business As Name:University of Tennessee Knoxville
  • Erik Zinser
  • (865) 974-9283
Award Date:07/27/2005
Estimated Total Award Amount: $ 402,564
Funds Obligated to Date: $ 402,564
  • FY 2005=$402,564
Start Date:01/01/2006
End Date:12/31/2010
Transaction Type:Grant
Awarding Agency Code:4900
Funding Agency Code:4900
CFDA Number:47.050
Primary Program Source:490100 NSF RESEARCH & RELATED ACTIVIT
Award Title or Description:Collaborative Research: Ecotypic Diversity and Adaptation of Prochlorococcus in the Stratified, HighTemperature Waters of the Western Pacific Warm Pool
Federal Award ID Number:0526072
DUNS ID:003387891
Parent DUNS ID:003387891

Awardee Location

Street:1331 CIR PARK DR
Awardee Cong. District:02

Primary Place of Performance

Organization Name:University of Tennessee Knoxville
Street:1331 CIR PARK DR
Cong. District:02

Abstract at Time of Award

In most tropical and subtropical ecosystems, the prokaryotic cyanobacteria Prochlorococcus plays a critical role in ecosystem structure and biogeochemistry because it is the numerically dominant photoautotrophic picoplankter. Although the worldwide distributions of Prochlorococcus are generally understood, the precise reasons for its overwhelming ecological success have remained elusive. This picture has recently become complicated by the discovery that Prochlorococcus is not monophyletic and that different genetic clades of Prochlorococcus have remarkably different distributions with depth and over oceanic basins. Thus, our understanding of factors that structure Prochlorococcus populations in the natural environment, and our ability to predict how this structure might respond to environmental changes, are limited. The PIs will address this by focusing on naturally occurring populations in the Western Pacific Warm Pool, an area where Prochlorococcus is known to dominate, but where there are no data on clade abundances. In addition to being a large region of the Pacific Ocean with significance to the global carbon cycle, the Western Pacific Warm Pool (WPWP) is of particular interest because it is typically highly stratified, with surface waters having extreme temperatures and light levels compared to those at depth. Populations of Prochlorococcus at the surface and at depth experience different environmental pressures, and may belong to different clades and have different adaptive physiologies. The PIs will test this hypothesis on a cruise from Christchurch, New Zealand to Hawaii through the stratified WPWP. Samples from this transect will be used to quantify (using quantitative PCR) the six known clades of Prochlorococcus and to search for new clades (using clone libraries and isolates) and their abundances. The ultimate goal is to relate clade abundances to temperature, light, nutrient concentrations and other measured biological, chemical and physical variables. This project will encompass multiple layers of outreach to scientists and the scientific community at large. Data and strains will be deposited at national repositories and results will be disseminated through publications, professional meeting presentations, and a project web site. Undergraduates and graduate students will be trained, and students will be an integral part of the data collection, analysis, and dissemination phases and will be encouraged to present at national meetings. Cross-institutional training will enhance graduate student education. The PIs will integrate results from this project into undergraduate and graduate curricula at their home institutions and will be attending the NSF sponsored 'Scientific Inquiry in the K-16 Classroom' seminar to develop methods to link results to primary education. The PIs will use presentations and activities with local outreach groups, such as the Hawaii Academy of Sciences, to encourage scientific understanding through mentoring, science symposia and science competitions for primary and secondary school students.

Publications Produced as a Result of this Research

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Zinser, ER, D Lindell, ZI Johnson, ME Futschik, C. Steglich, ML Coleman, MA Wright, T Rector, R Steen, N MAcNulty, LR Thompson, SW Chisholm "Choreography of the transcriptome, photophysiology, and cell cycle of a minimal photoautotroph, Prochlorococcus" PLoS ONE, v.4, 2009, p.e5135. doi:10.1371/journal.pone.0005135 

Zinser, ER; Johnson, ZI; Coe, A; Karaca, E; Veneziano, D; Chisholm, SW "Influence of light and temperature on Prochlorococcus ecotype distributions in the Atlantic Ocean" LIMNOLOGY AND OCEANOGRAPHY, v.52, 2007, p.2205. View record at Web of Science

Malmstrom, R.R.;Coe, A.;Kettler, G.C.;Martiny, A. C.;Frias-Lopez, J.;Zinser, E.R.;Chisholm, S. W.; "Temporal dynamics of Prochlorococcus ecotypes in the Atlantic and Pacific Oceans" ISME J., v.4, 2010, p.1252-1264.

Zinser, ER; Coe, A; Johnson, ZI; Martiny, AC; Fuller, NJ; Scanlan, DJ; Chisholm, SW "Prochlorococcus ecotype abundances in the North Atlantic Ocean as revealed by an improved quantitative PCR method" APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.72, 2006, p.723. doi:10.1128/AEM.72.1.723-732.200  View record at Web of Science

Moore, LR; Coe, A; Zinser, ER; Saito, MA; Sullivan, MB; Lindell, D; Frois-Moniz, K; Waterbury, J; Chisholm, SW "Culturing the marine cyanobacterium Prochlorococcus" LIMNOLOGY AND OCEANOGRAPHY-METHODS, v.5, 2007, p.353. View record at Web of Science

Johnson, ZI; Zinser, ER; Coe, A; McNulty, NP; Woodward, EMS; Chisholm, SW "Niche partitioning among Prochlorococcus ecotypes along ocean-scale environmental gradients" SCIENCE, v.311, 2006, p.1737. doi:10.1126/science.111805  View record at Web of Science

Morris, JJ; Kirkegaard, R; Szul, MJ; Johnson, ZI; Zinser, ER "Facilitation of robust growth of Prochlorococcus colonies and dilute liquid cultures by "Helper" heterotrophic bacteria" APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.74, 2008, p.4530. doi:10.1128/AEM.02479-0  View record at Web of Science

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