Award Abstract # 1332898
Collaborative Research: Dissolved Phosphorus Processing by Trichodesmium Consortia: Quantitative Partitioning, Role of Microbial Coordination, and Impact on Nitrogen Fixation

NSF Org: OCE
Division Of Ocean Sciences
Recipient: WOODS HOLE OCEANOGRAPHIC INSTITUTION
Initial Amendment Date: July 8, 2013
Latest Amendment Date: July 8, 2013
Award Number: 1332898
Award Instrument: Standard Grant
Program Manager: Henrietta Edmonds
hedmonds@nsf.gov
 (703)292-7427
OCE
 Division Of Ocean Sciences
GEO
 Directorate For Geosciences
Start Date: October 1, 2013
End Date: September 30, 2016 (Estimated)
Total Intended Award Amount: $606,678.00
Total Awarded Amount to Date: $606,678.00
Funds Obligated to Date: FY 2013 = $606,678.00
History of Investigator:
  • Benjamin Van Mooy (Principal Investigator)
    bvanmooy@whoi.edu
Recipient Sponsored Research Office: Woods Hole Oceanographic Institution
266 WOODS HOLE RD
WOODS HOLE
MA  US  02543-1535
(508)289-3542
Sponsor Congressional District: 09
Primary Place of Performance: Woods Hole Oceanographic Institution
260 Woods Hole Rd. MS 4
Woods Hole
MA  US  02543-1535
Primary Place of Performance
Congressional District:
09
Unique Entity Identifier (UEI): GFKFBWG2TV98
Parent UEI:
NSF Program(s): BIOLOGICAL OCEANOGRAPHY,
Chemical Oceanography
Primary Program Source: 01001314DB NSF RESEARCH & RELATED ACTIVIT
Program Reference Code(s): 1389, 1650, 1670, 4444
Program Element Code(s): 165000, 167000
Award Agency Code: 4900
Fund Agency Code: 4900
Assistance Listing Number(s): 47.050

ABSTRACT

Colonies of the cyanbacterium Trichodesmium are responsible for a large fraction of N2 fixation in nutrient-poor, open-ocean ecosystems, ultimately fueling primary production in both Trichodesmium and in the broader planktonic community. However, in some parts of the ocean, the scarcity of dissolved phosphorus limits rates of Trichodesmium N2 fixation. Trichodesmium colonies employ an arsenal of strategies to mitigate the effects of phosphorus limitation, and the consortia of epibiotic bacteria in the colonies may play a significant role in phosphorus acquisition.

In this study, researchers from Woods Hole Oceanographic Institution and Columbia University will use metagenomic and metatranscriptomic sequencing to investigate how phosphorus metabolism is coordinated in Trichodesmium consortia, and to discern the role of quorum sensing in phosphorus acquisition and partitioning. Results from this study are expected to expand understanding of Trichodesmium from a monospecific colony whose primary function is fixing CO2 and N2 toward a unique planktonic consortium with a diverse, complex, and highly coordinated overall metabolism that exerts profound control over the cycling of inorganic and organic nutrients in the oligotrophic upper ocean.

Broader Impacts: This project will provide research experience for an under-represented ethnic graduate student and take advantage of established K-12 standards-based outreach programs to increase ocean literacy in children and amongst the public.

PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH

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Van Mooy, B.A.S., Krupke, A., Dyhrman, S. T., Fredricks, H.F., Frischkorn, K.R., Ossolinski, J.E., Repeta, D. J. ,Rouco-Molina, M., Seewald, J.S., Sylva, S.P. "Major role of planktonic phosphate reduction in the marine phosphorus redox cycle" Science , v.348 , 2015 , p.783 10.1126/science.aaa8181
Andreas Krupke, Laura R.Hmelo, Justin E.Ossolinski, Tracy J. Mincer, and Benjamin A.S.Van Mooy "Quorum Sensing Plays a Complex Role in Regulating the Enzyme Hydrolysis Activity of Microbes Associated with Sinking Particles in the Ocean" Frontiers in Marine Science. , v.3 , 2016 10.3389/fmars.2016.00055
C. M. Hansel,C. Buchwald,J. M. Diaz, J. E. Ossolinski, S. T. Dyhrman, B. A. S. Van Mooy, D. Polyviou "Dynamics of extracellular superoxide production by Trichodesmium colonies from the Sargasso Sea" Limnology and Oceanography , v.61 , 2016 , p.1188 10.1002/lno.10266

PROJECT OUTCOMES REPORT

Disclaimer

This Project Outcomes Report for the General Public is displayed verbatim as submitted by the Principal Investigator (PI) for this award. Any opinions, findings, and conclusions or recommendations expressed in this Report are those of the PI and do not necessarily reflect the views of the National Science Foundation; NSF has not approved or endorsed its content.

The ocean plays an important role in regulating Earth's climate, primarily because the tiny plants that float near the surface, known as phytoplankton, consume the greenhouse gas carbon dioxide through photosynthesis.  Because carbon dioxide released from human activities is causing the Earth to warm, it is important to know how much carbon dioxide phytoplankton consume and what other factors impact this.   Nitrogen, the same stuff found in plant fertilizers, is necessary for phytoplankton to conduct photosynthesis, but nitrogen is scarce in the ocean.  One type of phytoplankton, called Trichodesmium, has a way of extracting its own nitrogen from air, which means that they play a special role in the ocean.  Trichodesmium lives in little colonies, or blobs about the size of the letter "o" in the text you are reading, which contain dozens of Trichodesmium along with hundreds of other microbes.  We found that all of these other microbes chemically communicate with each other by secreting ("oozing") molecules that tell their neighboring microbes who they are and what they need. In addition, we learned that one of the things they communicate about is delivering to Trichodesmium the other nutrients, such as phosphorus and iron, it needs to be able to make its own nitrogen.  This is a really significant finding because we now know that communication is important in determining how much nitrogen gets delivered to phytoplankton in the ocean, but before we thought that nutrients floating out to the ocean in dust were the main important factor.  It is amazing to think that communications between microbes ultimately affect how much carbon dioxide gets consumed by phytoplankton.  The next step is to figure out if this communication in the ocean is likely to increase or decrease in the future if Earth's climate continues to change.


Last Modified: 01/10/2017
Modified by: Benjamin Van Mooy

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