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Award Detail

Doing Business As Name:University of South Alabama
  • Jeffrey W Krause
  • (251) 861-2141
Award Date:08/20/2013
Estimated Total Award Amount: $ 513,777
Funds Obligated to Date: $ 513,777
  • FY 2013=$513,777
Start Date:01/01/2014
End Date:09/30/2019
Transaction Type:Grant
Awarding Agency Code:4900
Funding Agency Code:4900
CFDA Number:47.050
Primary Program Source:040100 NSF RESEARCH & RELATED ACTIVIT
Award Title or Description:Dimensions: Collaborative Research: Bacterial Taxa that Control Sulfur Flux from the Ocean to the Atmosphere
Federal Award ID Number:1342699
DUNS ID:172750234
Parent DUNS ID:172750234
Program:Dimensions of Biodiversity
Program Officer:
  • Michael Sieracki
  • (703) 292-7585

Awardee Location

Street:307 University Boulevard
Awardee Cong. District:01

Primary Place of Performance

Organization Name:University of South Alabama
Street:307 University Blvd
Cong. District:01

Abstract at Time of Award

This project is based on the globally important function of bacterial transformation of the ubiquitous organic sulfur compound dimethylsulfoniopropionate (DMSP) in ocean surface waters. Recent genetic discoveries have identified key genes in the two major DMSP degradation pathways, and the stage is now set to identify the factors that regulate gene expression to favor one or the other pathway during DMSP processing. The taxonomy of the bacteria mediating DMSP cycling has been deduced from genomic and metagenomic sequencing surveys to include four major groups in the ocean. Understanding how the regulation of DMSP degradation differs among these groups and maps to the relationships between these groups is important information for understanding the marine sulfur cycle and predicting its function in a changing ocean. The project will incorporate the use of model organism studies, microcosm experiments (at Dauphin Island Sea Lab, AL), and time-series field studies with an autonomous sample collection instrument (at Monterey Bay, CA). This project will ascertain how the major taxa of bacterial DMSP degraders in seawater regulate DMSP transformations, and address the implications of bacterial functional, genetic, and taxonomic diversity for global sulfur cycling.. The project will train graduate students and a post-doctoral scholar in microbial biodiversity and provide research opportunities and mentoring for undergraduate students. Students will obtain interdisciplinary training in microbial ecology, sulfur chemistry/biogeochemistry, and environmental bioinformatics, and will participate in research to improve our understanding of how marine bacterioplankton regulate the global fate of sulfur. An outreach program will enhance understanding of the role and diversity of marine microorganisms in global elemental cycles among promising high school students. Advanced Placement (AP) Biology students at a racially diverse Athens, GA, high school will participate in marine microbial research that covers key learning goals in the new AP Biology curriculum. Students will conduct classroom laboratory exercises with bacterial strains isolated from coastal Georgia seawater to learn ecological, microbiological, molecular biological, and bioinformatic concepts. During an end-of-the-year field trip to Sapelo Island, students will isolate new strains that become the study organisms for the next class of AP Biology students. Two high school students will be selected for summer research internships in the laboratories of the principal investigators. The project also supports education and outreach efforts at public aquaria (DISL Estuarium and Monterey Bay Aquarium) and summer graduate courses.

Publications Produced as a Result of this Research

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DeBose, J. L., Kiene, R. P., & Paul, V. J. "Eggs and larvae of Acropora palmata and larvae of Porites astreoides contain high amounts of dimethylsulfoniopropionate" Journal of Experimental Marine Biology and Ecology, v.473, 2015, p.146. doi:10.1016/j.jembe.2015.08.015 

Salgado, P., R. P. Kiene, W. J. Wiebe, and C. Magalhães. "Salinity as a regulator of DMSP catabolic pathways in Ruegeria pomeroyi DSS-3." Journal of Microbiology, v., 2014, p.. doi:10.1007/s12275-014-4409-1. 

Lizotte, M., Levasseur, M., Law, C., Walker, C., Safi, K., Marriner, A., & Kiene, R. P. "Dimethylsulfoniopropionate (DMSP) and dimethylsulfide (DMS) cycling across contrasting biological hotspots of the New Zealand Subtropical Front." Ocean Science, v.13, 2017, p.961. doi:10.5194/os-13-961-2017 

Motard-Côté, J., Rellinger, A., Kieber, D. J., & Kiene, R. P. "Influence of the Mississippi River plume and non-bioavailable DMSP on dissolved DMSP turnover in the northern Gulf of Mexico" Environmental Chemistry, v.13, 2015, p.280. doi:org/10.1071/EN15053 

Varaljay, V. A., J. Robidart, C. M. Preston, S. M. Gifford, B. P. Durham, J. P. Ryan, R. Marin III, R. P. Kiene, J. P. Zehr, C. A. Scholin, and M. A. Moran. "Single-Taxon Field Measurements of Bacterial Gene Regulation Controlling DMSP Fate" ISME Journal, v.9, 2015, p.1677. doi:10.1038/ismej.2015.23 

Galindo, V., M. Levasseur, M. Scarratt, C. J. Mundy, M. Gosselin, R. P. Kiene, M. Gourdal, and M. Lizotte. "Under-ice microbial dimethylsulfoniopropionate metabolism during the melt period in the Canadian Arctic Archipelago." Marine Ecology-Progress Series, v.524, 2015, p.39. doi:10.3354/meps11144 

Lavoie, M , Waller, J C, Kiene, R P, & Levasseur, M. "Polar marine diatoms likely take up a small fraction of dissolved dimethylsulfoniopropionate relative to bacteria in oligotrophic environments." Aquatic Microbial Ecology, v.81, 2018, p.213. doi: 

Mohapatra, B. R., Schambeau, L., Pannell, L. K., & Kiene, R. P. "Comparative proteomics of temperate and polar Phaeocystis species. In press" Nova Hedwegia., v.105, 2017, p.15. doi:10.1127/Nova_hedwigia/2016/0393 

del Valle, D. A., Martínez-García, S., Sañudo-Wilhelmy, S. A., Kiene, R. P., & Karl, D. M. "Methionine and dimethylsulfoniopropionate as sources of sulfur to the microbial community of the North Pacific Subtropical Gyre." Aquatic Microbial Ecology,, v.75, 2015, p.103. doi:10.3354/ame01750 

Motard-Côté, J., & Kiene , R. P. "Osmoprotective role of dimethylsulfoniopropionate (DMSP) for estuarine bacterioplankton" Aquatic Microbial Ecology, v.76, 2015, p.133. doi:10.3354/ame01772 

Tyssebotn, I M B, Kinsey, J D, Kieber, D J, Kiene, R P, Rellinger, A N, & Motard-Côté, J. "Concentrations, biological uptake, and respiration of dissolved acrylate and dimethylsulfoxide in the northern Gulf of Mexico." Limnology and Oceanography, v.62, 2017, p.1198. doi:10.1002/lno.10495 

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