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Award Detail

Doing Business As Name:University of Utah
  • Colin Dale
  • (801) 585-0558
  • Robert B Weiss
Award Date:08/06/2005
Estimated Total Award Amount: $ NaN
Funds Obligated to Date: $ 790,247
  • FY 2005=$763,935
  • FY 2007=$26,312
Start Date:10/01/2005
End Date:09/30/2011
Transaction Type:Grant
Awarding Agency Code:4900
Funding Agency Code:4900
CFDA Number:47.074
Primary Program Source:490100 NSF RESEARCH & RELATED ACTIVIT
Award Title or Description:Microbial Genome Sequencing: Comparative Whole Genome Sequencing of Grain Weevil Endosymbionts
Federal Award ID Number:0523818
DUNS ID:009095365
Parent DUNS ID:009095365
Program Officer:
  • Matthew Kane
  • (703) 292-7186

Awardee Location

Street:75 S 2000 E
County:Salt Lake City
Awardee Cong. District:02

Primary Place of Performance

Organization Name:University of Utah
Street:75 S 2000 E
County:Salt Lake City
Cong. District:02

Abstract at Time of Award

The major aim of this project is to determine the complete genome sequences of two closely related bacterial endosymbionts that live in obligate symbiosis with the grain weevils Sitophilus zeamais and Sitophilus oryzae. Although neither of these bacterial endosymbionts have been cultured in the laboratory, their DNA will be extracted directly from insect tissues and sequenced using automated, high-throughput procedures at the University of Utah Genome Center. The sequence data will be annotated, analyzed and made accessible to the public through the NCBI microbial genomes database. Comparative bioinformatic analyses will be performed on the complete endosymbiont genome sequences to understand the life histories and lifestyles of the weevil endosymbionts. In the context of the wide array of microbial life on our planet the weevil endosymbionts are important for a number of reasons. They have served as a model system in symbiosis research for over a century, with more than 100 scientific papers describing studies involving this system. Interaction between host and endosymbiont also will serve as a model for the study of degenerative evolutionary processes known to affect the genomes of both beneficial and harmful bacterial associates of animals and plants. Finally, the weevils themselves are major insect pests of stored grain products. Many billions of dollars are lost each year as a result of weevil infestation. The availability of the complete genome sequences of the weevil endosymbionts will provide an understanding of biological interplay between the weevil hosts and their bacterial partners. This should prove useful in the development of new pest control strategies. The current project offers numerous opportunities for the training of high-school students along with undergraduate and postgraduate university students in the fields of bioinformatics, genomics and evolution. Students will have opportunities to interact with scientists from a wide range of disciplines including biology, computer science and engineering.

Publications Produced as a Result of this Research

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Pontes, MH; Dale, C "Culture and manipulation of insect facultative symbionts" TRENDS IN MICROBIOLOGY, v.14, 2006, p.406. doi:10.1016/j.tim.2006.07.00  View record at Web of Science

Dale, C; Moran, NA "Molecular interactions between bacterial symbionts and their hosts" CELL, v.126, 2006, p.453. doi:10.1016/j.cell.2006.07.01  View record at Web of Science

Pontes, MH; Babst, M; Lochhead, R; Oakeson, K; Smith, K; Dale, C "Quorum Sensing Primes the Oxidative Stress Response in the Insect Endosymbiont, Sodalis glossinidius" PLOS ONE, v.3, 2008, p.. doi:10.1371/journal.pone.000354  View record at Web of Science

Pontes, MH; Smith, KL; De Vooght, L; Van den Abbeele, J; Dale, C "Attenuation of the Sensing Capabilities of PhoQ in Transition to Obligate Insect-Bacterial Association" PLOS GENETICS, v.7, 2011, p.. doi:10.1371/journal.pgen.100234  View record at Web of Science

Login, FH; Balmand, S; Vallier, A; Vincent-Monegat, C; Vigneron, A; Weiss-Gayet, M; Rochat, D; Heddi, A "Antimicrobial Peptides Keep Insect Endosymbionts Under Control" SCIENCE, v.334, 2011, p.362. doi:10.1126/science.120972  View record at Web of Science

Clark, AJ; Pontes, M; Jones, T; Dale, C "A possible heterodimeric prophage-like element in the genome of the insect endosymbiont Sodalis glossinidius" JOURNAL OF BACTERIOLOGY, v.189, 2007, p.2949. doi:10.1128/JB.00913-0  View record at Web of Science

Pontes, MH; Dale, C "Lambda Red-Mediated Genetic Modification of the Insect Endosymbiont Sodalis glossinidius" APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.77, 2011, p.1918. doi:10.1128/AEM.02166-1  View record at Web of Science

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