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Research Spending & Results

Award Detail

Awardee:HOWARD UNIVERSITY (INC)
Doing Business As Name:Howard University
PD/PI:
  • Preethi L Chandran
  • (202) 806-4759
  • preethi.chandran@howard.edu
Co-PD(s)/co-PI(s):
  • Sergei Nekhai
Award Date:07/10/2020
Estimated Total Award Amount: $ 499,959
Funds Obligated to Date: $ 499,959
  • FY 2020=$499,959
Start Date:07/15/2020
End Date:06/30/2023
Transaction Type:Grant
Agency:NSF
Awarding Agency Code:4900
Funding Agency Code:4900
CFDA Number:47.074
Primary Program Source:040100 NSF RESEARCH & RELATED ACTIVIT
Award Title or Description:Excellence in Research: Biophysical mechanism by which mannose and N glycans modifies and protects biological surfaces
Federal Award ID Number:2000175
DUNS ID:056282296
Parent DUNS ID:056282296
Program:Molecular Biophysics
Program Officer:
  • Engin Serpersu
  • (703) 292-7124
  • eserpers@nsf.gov

Awardee Location

Street:2400 Sixth Street N W
City:Washington
State:DC
ZIP:20059-9000
County:
Country:US
Awardee Cong. District:00

Primary Place of Performance

Organization Name:Howard University
Street:1011 LK Downing Hall 2300 6th St
City:Washington
State:DC
ZIP:20059-0001
County:Washington
Country:US
Cong. District:00

Abstract at Time of Award

The surface of pathogens is shielded by short polymers of sugars known as glycans. Glycans mask pathogens and signature molecules from host cells and confound therapeutic treatments. N-glycans are the commonly found type of glycans found on cell surfaces. Pathogens like Ebola, SARS, HIV, and COVID 19 are shielded by N-glycans having high mannose content, with the mannose content sometimes increasing during the host infection. The goal of the proposal is to investigate what biophysical properties of mannose residues present in shields of N-glycans confer protection of pathogens against the host immune system. This project will train graduate and undergraduate students and provide an annual hands-on STEM workshops to engage high school students in research and everyday science. Understanding how mannose residues presented in N-glycans protect pathogens will help us strategically disarm the glycan shield fortress, thereby making pathogens more vulnerable to detection, sanitizing, and treatment. Despite the ubiquitous presence of glycan sugars on biological surfaces, little is known currently about how glycans steer interfacial effects like aggregation, biofilm formation, charge shielding, antifouling, immune-stealth, and transport through mucus. This project will lead to a better understanding of the biophysics of mannose residues when presented in N-glycan architecture along with non-mannose sugars, and how they relate to the improved solubility and aggregation, mucous penetration, and immune evasion of glycosylated molecules. To understand the uniqueness of mannose biophysics and how it interplays with other N-glycan sugars, the cross- and self- interactions of all N-glycan sugars will be determined, and rules for integrating single-sugar biophysics to glycosylated system behavior will be evaluated. A pseudo-typed HIV virus is the controlled sugar-presentation platform. These studies will synergistically link observations from force-spectroscopy, rheology, and dynamic light scattering, along with perturbations from glycosidase and lectin addition. This project is jointly supported by the Historically Black Colleges and Universities (HBCU) Excellence in Research program and the Molecular Biophysics program in the Molecular and Cellular Biosciences Division. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

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